PrimeEditing
Prime Editing Template Generator (RTT/PBS)
Break down RTT and PBS logic with a purpose-built template builder.
Build RTT and PBS templates with stability guardrails and mismatch previews in Helix Studio, the Genome IDE for prime editing design.
System of Record →Section 1
What the tool is
The Template Generator visualizes RTT and PBS segments, showing exactly where mismatches and edits sit relative to the nick.
Section 2
Why scientists care
RTT/PBS selection is often trial-and-error, leading to unstable priming or edits outside the window.
Section 3
How Helix solves it
Interactive RTT/PBS sliders with guardrails for GC%, Tm, and length
Section 4
How the algorithm works
Templates are scored on priming stability, mismatch placement, and proximity to the nick site.
Section 5
Try it in Helix Studio
Load your desired edit, slide RTT/PBS lengths, and lock in the template with the highest stability badge.
Section 6
FAQ
What RTT/PBS ranges do you support?
Common ranges (8–20 nt PBS, 10–40 nt RTT) are supported with custom overrides when needed.
Can I preview secondary structure?
We flag strong hairpins or homopolymers and suggest alternates before export.
Do you handle reverse complements?
Yes—templates are oriented automatically based on target strand and nick direction.