PrimeEditing
pegRNA Checker & Validator
QC pegRNAs before synthesis: length, stability, and off-target context.
QC pegRNAs for length, stability, nick alignment, and off-target context before synthesis inside Helix Studio, the Genome IDE for Prime Editing.
System of Record →Section 1
What the tool is
The pegRNA Checker applies a QC gauntlet to every candidate—flagging stability risks, off-target liabilities, and design rule violations.
Section 2
Why scientists care
Prime editing failures often trace back to small template or nicking misalignments caught too late.
Section 3
How Helix solves it
Length, GC, Tm, and secondary-structure checks with severity tiers
Section 4
How the algorithm works
QC rules blend thermodynamic models, heuristics from published PE datasets, and Helix’s off-target scoring.
Section 5
Try it in Helix Studio
Import pegRNAs or generate them in Helix, run validation, and fix any flagged items instantly.
Section 6
FAQ
Can I set custom QC thresholds?
Yes—tune GC/Tm ranges, allowed offsets, and structural thresholds per project.
Do you validate nicking guide strands?
We ensure nicking sgRNAs are on the correct strand and distance for PE3/PE5 strategies.
Are QC results exportable?
Export PDF/CSV summaries or keep them in Helix for traceability.