CRISPR
CRISPR Off-Target Prediction Engine
Cas9 off-target modeling with GPU-ready kernels for deeper, faster scans.
Run Cas9 policy gated off-target risk enumeration with genome-wide sweeps, context-aware scoring, and GPU-ready kernels inside Helix Studio, the Genome IDE for editing.
System of Record →Section 1
What the tool is
The policy gated off-target risk enumeration Engine ranks potential cut sites across the genome and surfaces the ones that matter for your experiment.
Section 2
Why scientists care
Off-target checks are often bolted-on scripts run after design, leaving risky guides in circulation.
Section 3
How Helix solves it
Genome-wide k-mer/seed scans tuned per nuclease to surface plausible off-site matches
Section 4
How the algorithm works
Initial scans run CPU-side with aggressive pruning; high-risk candidates trigger deeper alignments.
Section 5
Try it in Helix Studio
Paste your guide set, run a sweep, and drop safe guides straight into the design queue.
Section 6
FAQ
Do you support bulge-aware scoring?
Yes—bulge-tolerant alignments are included; GPU kernels will accelerate these paths next.
Can I prioritize off-targets near specific genes?
Tag loci of interest and Helix boosts their weighting in the final rank.
How are results delivered?
As ranked tables with genome coordinates, annotations, and quick links back to the visual genome track.