CRISPR
CRISPR Multiplex Planning Tool
Simulate interactions between multiple gRNAs before you build the construct.
Plan multiplex CRISPR designs, detect guide collisions, and model combined off-target risk in Helix Studio, a Genome IDE built for complex edits.
System of Record →Section 1
What the tool is
The Multiplex Planning Tool orchestrates guide sets, checking distance, frame impacts, and shared off-target liabilities as you build constructs.
Section 2
Why scientists care
Multiplex designs explode in complexity—guide collisions, shared off-targets, and delivery constraints derail experiments.
Section 3
How Helix solves it
Visual lane for each guide with distance checks and frame awareness
Section 4
How the algorithm works
Guides are simulated together with additive risk scoring and collision detection for overlapping PAMs or spacers.
Section 5
Try it in Helix Studio
Drop your target region, stack guides, and watch Helix auto-check spacing and conflicts.
Section 6
FAQ
How many guides can I plan at once?
We support typical small pools (2–20 guides) with performance headroom for more depending on sequence length.
Can I mix nucleases?
Yes—define per-guide nuclease settings and Helix applies the right PAM and cut rules.
Do you model delivery constraints?
We include promoter/terminator footprints and payload size estimates to keep constructs feasible.